abstract：:Native tetrameric alpha 2-macroglobulin molecules (alpha 2M) can be converted into a population of dimers by incubation with various divalent cations such as Zn, Cd, Mg, Cu, Ni, Co. This dissociation is completed within 30 min at 37 degrees C. These dimers have a characteristic shape and a size of about 16 X 8 nm, and...

journal_title：European journal of cell biology

authors： Couture-Tosi E,Tapon-Bretaudière J,Pochon F,Barray M,Bros A,Delain E

更新日期：1986-12-01 00:00:00

abstract：:Cytoskeletal components backing the brush border of the rat kidney proximal tubule cell were identified and compared with those of the well characterized intestinal brush border by immuneoverlay and immunocytochemistry. Antibodies reactive against the intestinal microvillus core components, villin and fimbrin, as well...

journal_title：European journal of cell biology

authors： Rodman JS,Mooseker M,Farquhar MG

更新日期：1986-12-01 00:00:00

abstract：:In the presence of various commonly used buffers, phosphate-buffered saline (PBS), tris-buffered saline (TBS), Na-cacodylate, bovine serum albumin and a wide range of cytochemically active proteins (monoclonal and polyclonal IgG, concanavalin A, Ricinus communis lectin I, Helix pomatia lectin, protein A) were complexe...

journal_title：European journal of cell biology

authors： Lucocq JM,Baschong W

更新日期：1986-12-01 00:00:00

abstract：:Cultured fetal rat hepatocytes derived from 12, 15 and 19-day gestation rats are capable of secreting transferrin. When dexamethasone is added to the medium an increased secretion rate is observed. The changes in secretion rates in control as well as dexamethasone-treated cells during culture have been shown to correl...

journal_title：European journal of cell biology

authors： Kraemer M,Vassy J,Brighton V,Fuller S,Yeoh G

更新日期：1986-10-01 00:00:00

abstract：:Oocyte-thymocyte mouse cell hybrids were produced using polyethylene glycol (PEG) and examined at the ultrastructural level. Fusion was accomplished either before or after activation of metaphase II oocytes. In both experimental variants thymocyte nuclei undergo remodelling which comprises the following sequence of ev...

journal_title：European journal of cell biology

authors： Szöllösi D,Czołowska R,Sołtynska MS,Tarkowski AK

更新日期：1986-10-01 00:00:00

abstract：:The interaction between myelin basic protein (MBP) and G-actin was studied under nonpolymerizing conditions, i.e.,2mM HEPES, pH 7.5, 0.1 mM CaCl2 and 0.2 mM ATP. Fluorescence studies using pyrenyl-actin and the measurements of ATP hydrolysis rate show that MBP induces changes in the structure of the actin monomer simi...

journal_title：European journal of cell biology

authors： Dobrowolski Z,Osińska H,Mossakowska M,Baryłko B

更新日期：1986-10-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) infection causes a rapid, progressive disruption of the host cell cytoskeleton that correlates with actin depolymerization. Whole-mount (3D) electron microscopy was used to analyze the cytoskeleton of uninfected and HCMV-infected human lung fibroblast cells. Within 2 min of HCMV infection,...

journal_title：European journal of cell biology

authors： Jones NL,Lewis JC,Kilpatrick BA

更新日期：1986-08-01 00:00:00

abstract：:Human peripheral blood monocytes were obtained in very pure preparations by using buoyant density gradient centrifugation followed by velocity sedimentation. We introduced several modifications in the experimental procedure in order to take advantage of the minimal differences in cell density and size between monocyte...

journal_title：European journal of cell biology

authors： Sitar G,Mascheretti P,Pesce G,Fornasari P,Peveri G

更新日期：1986-04-01 00:00:00

abstract：:The presence of a filamin-like protein in oxyntic cells was established by indirect immunofluorescence microscopy. The location of this protein and myosin was studied, using specific antibodies, on frozen sections and isolated cells. Antifilamin and antimyosin reacted strongly with the luminal cytoplasm of the cells. ...

journal_title：European journal of cell biology

authors： Dabiké M,Munizaga A,Koenig CS

更新日期：1986-04-01 00:00:00

abstract：:The isolation and characterization of highly purified and structurally well-preserved peroxisomes from the renal cortex of different mammalian species (beef, sheep, and cat) is reported. Renal cortex tissue was homogenized and a peroxisome-enriched light mitochondrial fraction was prepared by differential centrifugati...

journal_title：European journal of cell biology

authors： Zaar K,Völkl A,Fahimi HD

更新日期：1986-03-01 00:00:00

abstract：:Polycationic derivatives of ferritin containing primary amino groups (CFah) or tertiary amino groups (CFdmp) were potent platelet agonists inducing shape change, aggregation and secretion, but also agglutination in the presence of EDTA. Pretreatment of platelets with neuraminidase, PGE1, indomethacin, or creatine kina...

journal_title：European journal of cell biology

authors： Patscheke H,Dierichs R

更新日期：1986-03-01 00:00:00

abstract：:Embryonic chick myoblasts possess an extensive network of cytoplasmic microtubules which emanate from a single, perinuclear centrosome containing a microtubule-organizing center (MTOC) and the centrioles. However, after myoblasts fuse into myotubes the centrosome is no longer apparent, and instead long parallel arrays...

journal_title：European journal of cell biology

authors： Connolly JA,Kiosses BW,Kalnins VI

更新日期：1986-01-01 00:00:00

abstract：:Monoclonal antibodies were used to define cell surface antigens which are present on rat hepatocytes but are absent from hepatoma cells. One monoclonal antibody, referred to as Be 9.2, recognizes a major component of purified rat liver plasma membranes with a Mr of 110 000. This antigen (gp110) was not found in the tr...

journal_title：European journal of cell biology

authors： Becker A,Neumeier R,Park CS,Gossrau R,Reutter W

更新日期：1986-01-01 00:00:00

abstract：:Studies on Ehrlich ascites tumor cells enriched in different cell cycle compartments by centrifugal elutriation have shown that after exclusion of oxygen, G1- and early S-phase cells are restricted from further cell cycle progression. On addition of a balanced mixture of deoxypyrimidine and deoxypurine nucleosides to ...

journal_title：European journal of cell biology

authors： Löffler M

更新日期：1985-11-01 00:00:00

abstract：:The transition in mouse teratocarcinomas of pluripotential stem cells to histogenetically determined ones of the neurogenic cell lineage was analysed at the total cellular protein level by two-dimensional gel electrophoresis. The change in morphology and function was found to be reflected by extensive shifts in the pr...

journal_title：European journal of cell biology

authors： Blüthmann H

更新日期：1985-09-01 00:00:00

abstract：:Rabbit antisera against homogeneous rat liver thioredoxin and thioredoxin reductase (NADPH-oxidized thioredoxin oxidoreductase, E.C. 1.6.4.5) were prepared and used for immunohistochemical analysis in adult rats. Immunoreactive thioredoxin and thioredoxin reductase were widely distributed in tissues and organs, but va...

journal_title：European journal of cell biology

authors： Rozell B,Hansson HA,Luthman M,Holmgren A

更新日期：1985-07-01 00:00:00

abstract：:Myeloid hematopoietic precursor cells are induced to differentiate by the macrophage and granulocyte differentiation-inducing protein MGI-2 (DF). This differentiation-inducing protein bound to double-stranded but not to single-stranded mammalian DNA. The bound MGI-2 was not eluted by high salt, but was eluted by sodiu...

journal_title：European journal of cell biology

authors： Weisinger G,Korn AP,Sachs L

更新日期：1985-05-01 00:00:00

abstract：:Morphological explanations for the effects of cytochalasin B (CB) on the digestive vacuole system of Paramecium caudatum were sought using electron-microscopic studies. Cytochalasin B (0.3 mM) essentially stopped vacuole release without stopping vacuole growth. Acidosome fusion with the new vacuoles was inhibited and ...

journal_title：European journal of cell biology

authors： Allen RD,Fok AK

更新日期：1985-05-01 00:00:00

abstract：:The subcellular site of accumulation of non-esterified cholesterol and sphingomyelin in lipid-laden livers and spleens from rats given multiple intravenous injections with liposomes made up of these lipids were investigated by morphological and biochemical techniques. The subcellular fractionation of liver homogenates...

journal_title：European journal of cell biology

authors： Bhuvaneswaran C,Venkatesan S,Mitropoulos KA

更新日期：1985-05-01 00:00:00

abstract：:The tumor-promoting drug 4 beta-phorbol 12-myristate 13-acetate (TPA) causes the loss of myofibrils in primary cultures of chick embryonic myotubes [9]. When myofibrils in chick myotubes are dispersed by TPA treatment (5 X 10(-8) M), there remains a class of non-myofibrillar actin filaments that are sensitive to subse...

journal_title：European journal of cell biology

authors： Connolly JA,Graham AJ

更新日期：1985-05-01 00:00:00

abstract：:The receptor-mediated endocytosis and intracellular processing of transferrin and mannose receptor ligands were investigated in bone marrow-derived macrophages, fibroblasts and reticulocytes. Mannosylated bovine serum albumin (BSA) conjugated to colloidal gold (Au-man-BSA) or colloidal gold-transferrin (AuTf) were use...

journal_title：European journal of cell biology

authors： Harding C,Levy MA,Stahl P

更新日期：1985-03-01 00:00:00

abstract：:Cell cycle kinetics and energy metabolism of Ehrlich ascites tumor cells grown in glucose-deprived medium supplemented with uridine, were investigated in order to extend our knowledge about the significance of the metabolic conversion of glucose for cell cycle progression of these cells. Viability (dye exclusion test)...

journal_title：European journal of cell biology

authors： Linker W,Löffler M,Schneider F

更新日期：1985-03-01 00:00:00

abstract：:The mutation raised (rsd, 3-95.4) of Drosophila melanogaster causes flightlessness as a consequence of abnormalities in the fibrillar flight muscles (FFMs). In this muscle type actin III is neither synthesized nor accumulated while adult tubular muscles of rsd flies are indistinguishable from wildtype. This paper demo...

journal_title：European journal of cell biology

authors： Lang AB,Reinhardt CA,Eppenberger HM,Wyss C

更新日期：1985-01-01 00:00:00

abstract：:Intact follicles as well as defolliculated oocytes of the mouse were studied by freeze-fracture electron microscopy. In intact follicles the oocyte plasma membrane shows two prominent types of intra-membrane particle array:gap junctions and yet undescribed rhombic particle arrays. The gap junctions vary in size (from ...

journal_title：European journal of cell biology

authors： van den Hoef MH,Bluemink JG,de Laat SW

更新日期：1984-11-01 00:00:00

abstract：:Dipyridamole (DPD), a widely used anti-thrombotic agent, inhibits in vitro directional migration of 10T1/2 mouse embryo cells in a dose dependent way. The inhibition is detectable at DPD concentrations comparable to those in human plasma after oral intake of the drug. Using a model for quantitative shape change analys...

journal_title：European journal of cell biology

authors： Grutman G,Verschueren H

更新日期：1984-11-01 00:00:00

abstract：:A method using proteins covalently coupled to glass surfaces has been applied to studies on the differentiation of neuroblasts. Neuroblastoma cells from clones N 18 and NIE 115 adhere to surfaces coated with fibronectin or laminin and extend rapidly growth cone-containing neurites. Some lectin-coated surfaces are also...

journal_title：European journal of cell biology

authors： Jousimaa J,Merenmies J,Rauvala H

更新日期：1984-09-01 00:00:00

abstract：:The muscle membrane of slow and fast fibers in cruralis and iliofibularis muscles and of intermediate fibers in submaxillaris muscle of the frog is studied in freeze-fracture replicas. A comparison of membrane folds, number, size and distribution of caveolae and of intramembrane particles (IMP) is given. In slow muscl...

journal_title：European journal of cell biology

authors： Verma V

更新日期：1984-09-01 00:00:00

abstract：:Microbodies containing bipyramidal crystalline nucleoid inclusions occur within every cortical cell in roots of Yucca torreyi. Reaction product deposition attributable to catalase, glycolate oxidase, and urate oxidase activities are cytochemically localized to Yucca root microbodies and classifies them as unspecialize...

journal_title：European journal of cell biology

authors： Kausch AP

更新日期：1984-07-01 00:00:00

abstract：:Leydig cells in developing fetal pig testis contained during the fetal regressive phase large accumulations of intermediate filaments. Before and after this period these filaments were arranged in a criss-cross fashion. In the pig as well as in the dog testis these filaments have been characterized as vimentin. Within...

journal_title：European journal of cell biology

authors： van Vorstenbosch CJ,Colenbrander B,Wensing CJ,Ramaekers FC,Vooijs GP

更新日期：1984-07-01 00:00:00

abstract：:Monensin, a monovalent ionophore, caused swelling of mature cisternae of plant Golgi apparatus. The appearance of swollen cisternae was time-dependent and linear over a period of 1 h with an estimated maximum rate of production of one swollen cisterna every 3 to 4 min. Implicit in these observations was a need for the...

journal_title：European journal of cell biology

authors： Boss WF,Morré DJ,Mollenhauer HH

更新日期：1984-05-01 00:00:00

abstract：:This study reports the ultrastructural detection of fucosyl residues at the surface of axenically grown roots of Zea mays. The method used involved sequential binding of UeA lectin to the root and coupling of the bound UeA with fucosyl ferritin. Superficial dense ferritin labelling was found in the slime droplet enshe...

journal_title：European journal of cell biology

authors： Rougier M

更新日期：1984-05-01 00:00:00

abstract：:The uptake mechanism of homologous IgG and immune complex, and the participation of coated vesicles in this process were studied in rat peritoneal macrophages. Peroxidase-antiperoxidase (PAP) immune complex produced in rat, and purified rat IgG adsorbed to gold particles (IgG-Au) were used as ligands. Freshly collecte...

journal_title：European journal of cell biology

authors： Kiss AL,Röhlich P

更新日期：1984-05-01 00:00:00

abstract：:Triton-treated cells of the coenocytic green alga, Ernodesmis verticillata, manifest longitudinal contraction and centripetal closure of the cytoplasm in the presence of exogenous free calcium ions. Complete closure of the cytoplasm around the vacuolar remnants is obtained with 1 mM ATP present in the activation mediu...

journal_title：European journal of cell biology

authors： La Claire JW2nd

更新日期：1984-03-01 00:00:00

abstract：:Formation of the complete spindles during the budding process of Saccharomyces uvarum was investigated by fluorescence microscopy of protoplasted cells. Protoplasts were treated with anti-tubulin antibodies and DAPI, a fluorescent dye staining DNA. Thus, both chromatin and spindles could be visualized. Duplication as ...

journal_title：European journal of cell biology

authors： Baumstark-Khan C,Rink H,Zimmermann HP

更新日期：1984-01-01 00:00:00

abstract：:It was previously shown that avidin, a glycoprotein secreted in vivo by chicken oviduct, is produced by cultured transformed or damaged chicken embryo fibroblasts [27]. This report demonstrates synthesis and secretion of large amounts of avidin by macrophages isolated from chicken yolk sac. Avidin was secreted to the ...

journal_title：European journal of cell biology

authors： Korpela J

更新日期：1984-01-01 00:00:00

abstract：:This electron microscopic study demonstrates that formation of a functional eukaryotic 40S initiation complex is accompanied by conformational changes which obscure the characteristic structural features of the 40S ribosomal subunits and of the initiation factor eIF-3, the only macromolecular components of the complex...

journal_title：European journal of cell biology

authors： Boublik M,Hellmann W,Staehelin T,Trachsel H

更新日期：1983-11-01 00:00:00

abstract：:The Semper cells in the ommatidia of the blowfly contain an impressive cytoskeleton consisting of hundreds of microtubules. The microtubules are intimately interwoven by an extensive system of the smooth endoplasmic reticulum. The cells were permeabilized by saponin treatment, subsequently incubated in a loading mediu...

journal_title：European journal of cell biology

authors： Walz B

更新日期：1983-11-01 00:00:00

abstract：:Rhodamine-conjugated phalloin, a derivative of phalloidin which binds to F-actin, was associated with three types of structures in uredospore germlings of the bean rust fungus, Uromyces phaseoli. The structures were filaments, peripheral plaques, and intranuclear inclusions. The filaments, located throughout the germ ...

journal_title：European journal of cell biology

authors： Hoch HC,Staples RC

更新日期：1983-11-01 00:00:00

abstract：:Chlorpromazine (CPZ) at minimally effective concentrations accumulates mammalian cells in mitosis without lethal effects on the cells. Star-metaphase morphology similar to effects seen with classical antimitotic compounds probably results from the preferential action of CPZ on a specific class of microtubules--the pol...

journal_title：European journal of cell biology

authors： Boder GB,Paul DC,Williams DC

更新日期：1983-09-01 00:00:00

abstract：:The relative distribution of heparan sulfate-glycosaminoglycan (HS-GAG) and chondroitin sulfate-glycosaminoglycans (CS-GAG) of the mesangial matrix (MM) and the glomerular basement membrane (GBM), which represent the two glomerular extracellular matrices, was determined by a combination of enzymatic treatments and aut...

journal_title：European journal of cell biology

authors： Kanwar YS,Jakubowski ML,Rosenzweig LJ

更新日期：1983-09-01 00:00:00